Even though mastitis control programs are frequently used in dairy production, mastitis are still one of the most frequently occurring and costly diseases in modern dairy farms (Halasa et al., 2007). Mastitis monovalent vaccines are widely used in some countries showing significant results in the reduction of severity of clinical signs and duration of infection of environmental mastitis (Wilson et al., 2009). The goal of this study was to evaluate the impact in milk quality, fertility and economic outcome of a polyvalent vaccination protocol for mastitis STARTVAC®, HIPRA, Spain.